Nexera UC
Supercritical Fluid Extraction / Supercritical Fluid Chromatograph
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Supercritical Fluid Extraction/Chromatograph System
Preparative scale Nexera™ UC Prep is Here
Advances in column technology have led to a renewed interest in supercritical fluid chromatography (SFC), which uses a supercritical fluid as mobile phase. Compared to liquid, supercritical fluids have low viscosity and high diffusivity. By combining SFC with Supercritical fluid extraction (SFE), which utilizes the high permeability and diffusivity of supercritical fluids, the extraction and analysis of target components from solid samples can be automated. The new analytical technology “Unified Chromatography” improves the analytical workflow by integrating sample extraction, analysis by various separation modes, and high-sensitivity detection.
Funzionalità
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The analysis time can be effectively shortened because an excellent chromatographic resolution can be maintained even at higher flow rates than those typically used for HPLC.
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Separation results can be quickly obtained during method development by screening with both SFC and UHPLC.
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By using a fraction collector, small-volume fractionation is also possible (ex:1.5 mL vials).
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Target compounds are extracted from solid samples, and then automatically transferred to SFC.
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By using SFC, the consumption of organic solvent is reduced by 94.2%, compared to the normal-phase.
Videos
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Nexera UC Supercritical Fluid Extraction/Chromatograph System Narration Eddition
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Overview of the analytical technologies using supercritical fluid
Green Analytical Chemistry (GAC) is a concept of designing analytical methods that are friendly to both chemists and environment. It is necessary for the GAC-based methods to retain the same analytical performance (e.g., accuracy, sensitivity) as those by their conventional counterparts. Large solvent consumption is particularly concerning in a food analysis where complicated sample extraction and chromatography are routinely practiced. Supercritical fluid is a state of matter just like gas or liquid, and the characteristics of CO2 supercritical fluid are nonflammability, easy handling, and low toxicity, which are relevant to the operator safety. This webinar will show the characteristics of supercritical fluids CO2, the practicality of analytical techniques using it, and the applicability to GAC.
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Method development and Identification of Triacylglycerols species with SFC
Analyzing Triacylglycerols (TAGs) is essential to evaluate the quality and functionality of oils and fats. Chromatographic separation by typical methods that include GC and HPLC might be challenging due to a large number of TAGs species. Supercritical Fluid Chromatography (SFC) is one chromatography method that uses supercritical fluid (i.e. supercritical carbon dioxide) as a mobile phase. Here, we introduce the workflow of method scouting of SFC using Method Scouting Solutions and how to establish the optimized analytical method for TAGs species in natural oils and fats.
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