Chromatography

Ultra-High Speed

UFsweeper™ technology efficiently accelerates ions out of the collision cell, dramatically minimizing cross talk and reducing MRM analysis to the shortest time possible.

Fusion ofUltra Fast MRM and Ultra Fast Polarity Switching of 15msec

Shimadzu's Nexera UHPLC realizes extreme chromatographic resolution. When coupled with the Nexera, the LCMS-8030 can provide reliable and accurate detection of peaks only one second wide, maximizing UHPLC performance. Combined with a polarity switching time of just 15 msec, the next-generation of Ultra Fast triple quadrupole is realized.

Standard Chromatogram of 226 Pesticides in Two Minutes

An Ultra Fast Scan Speed of 15,000 u/sec

In product ion scan measurement (automatic MSMS, or synchronized survey scan) using MRM as the trigger, mass spectra of a precursor ion can be obtained during an MRM transition. Compound structure can be simultaneously confirmed by the resulting product ion spectra due to the high selectivity achieved during MRM with no interference from coeluting substances.

The figure below illustrates a 1.5-minute elution of 29 pesticides using the LCMS-8030 with the Nexera UHPLC system. Ultra Fast polarity switching and Ultra Fast MRM transitions deliver reliable and accurate quantitative results. Furthermore, the synchronized survey scan technology, utilizing a high-speed scanning rate of 15,000 u/sec, allows full spectrum scans within a series of MRM measurements, providing confirmation of target compounds with information-rich product ion spectra.

MRM transition and product ion scan measurement triggered by the MRM transition

500 MRM Transitions in One Second is Now Possible!

In the UFsweeper™ collision cell, there is no ion loss even at a 1 msec dwell time as ions are accelerated from the pressurized collision cell without losing momentum. In addition, higher RF power capability minimizes the pause time between each MRM transition. For the first time, true high-throughput analysis without sacrificing ion intensity is a reality.

Dwell time	%RSD, n=6
100msec	0.48
10msec	0.79
1msec	1.92

Alprazolam m/z 309 >281, FWHM＝3sec, Pause time 1msec, n=6